Neoderm®-M/B
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Composition

  • Epidermis
    • Human keratinocytes & melanocytes
  • Dermis
    • Fibroblasts _ Collagen matrix

Characteristics

  • Skin model composed of living cells, with the possibility ofcell metabolism and response measurement.
  • Melanocytes reside in the basal layer, and is structured similar to human skin including a full layer of epidermis and dermis (Figure 1).
  • A lighter and darker model can be chosen depending upon the level of melanin synthesis in melanocyte, Neoderm-M (Figure 2) and Neoderm-B (will be ready soon).
  • It can be used to evaluate changes in melanin content in the skin following ultraviolet ray exposure or drug/cosmetics applications.
  • It can also be used to study the mechanism of melanogenesis by ultraviolet ray exposure or by hormones such as MSH (melanocyte-stimulating hormone) and ACTH (adrenocorticotropic hormone).

Applications

  • Lightening test
    • Fontana-Masson stain / DOPA stain
    • Melanin content assay
    • Tyrosinase activity
  • Melanogenesis Research
    • Measurement of changes in melanin synthesis
      by hormones, cytokines or inhibitors
  • Irritation Test
    • Cell viability (MTT), IL-1α or PGE2 (ELISA)
  • Permeation Test
    • Barrier function & Permeation test (HPLC)
  • Skin Regeneration & Elasticity Test
    • Histological and morphological changes (H&E staining)
    • Proliferation rate of keratinocytes (MTT, IF)
    • Analysis of secreted proteins in keratinocytes
      (ELISA, Western blot, PCR, IHC)
    • Collagen synthesis (ELISA, IF)
  • Wound Healing Assay
    • Analysis of healingeffect in wound healing model (H&E staining)
  • Other molecular biological analysis
    • Proteomics and RNA array analysis

Well Type

6-well, 12-well

TEGO SCIENCE provides Contract Research Services using Neoderm®-M and Neoderm®-B